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CASA system · Multi-species

Reliable, repeatable and downloadable semen analysis

All-in-one CASA system for semen quality evaluation in bovine, equine and canine. Integrates microscope, camera and artificial vision software in a single workflow.

R²=0,99

Validated accuracy

Correlation vs NucleoCounter®, reference standard in sperm concentration

<2 min

Per sample

From loading to exportable report in Excel or PDF

20

Samples in 5 min

With optional AS-10 automatic stage, sequential analysis without intervention

3

Species

The context

Why it matters how you analyse semen

In many laboratories, semen analysis still depends on the technician’s judgement and manual methods. This creates specific problems that affect the quality of results and the credibility of data.

01

Inter-operator variability. Each technician obtains different results with the same sample, making it difficult to compare results

02

Long time at the microscope. Manual analysis consumes the time of specialised personnel who could be dedicated to higher-value tasks.

03

Data difficult to defend. Manual management in spreadsheets adds risk of error and complicates reproducibility in publications and audits.

04

Subjective morphology. Visual classification of abnormal forms varies by observer, especially in anomalies such as DMR or agglutination area.

Inter-operator variability · same ejaculate

Spermalyze
0%
Technician A
0%
Technician B
0%
Technician C
0%
Total motility · % motile

Spermalyze produces the same result in every analysis,
regardless of the operator.

The system

Spermalyze, all-in-one CASA system

Spermalyze integrates a heated stage microscope, 5-megapixel camera and proprietary software based on artificial vision and machine learning. Designed for routine ejaculate analysis in bovine, equine and canine, with algorithms trained specifically for each species.

01

Accurate and fast

Identifies and tracks each spermatozoon cell by cell in seconds using artificial intelligence, accounting for the particularities of each species.

  • 5 MP real camera · up to 300M pixels per image
  • 60 frames per second processing
  • Accepts egg-yolk extenders
  • Precise concentration even in undiluted straws
  • Detects spermatozoa adherent to epithelial cells

02

Integrated data management

The software is designed to simplify the management of all relevant information: animals, operators, doses and campaigns in a single database.

  • Re-analyse past videos without repeating the analysis
  • Custom labels and comments per analysis
  • Compare data across campaigns, centres and operators
  • Integrable with other databases and management systems
  • Export to Excel and PDF

03

Versatile and configurable

Combinable modules with algorithms trained for each species. Parameters configurable by breed. Permission levels per working mode.

  • Species- and breed-specific algorithms
  • Quality alerts and focus control
  • Warnings when sample does not meet configured thresholds
  • Compatible with multiple microscopes and video cameras
  • Reports customisable in format and parameters

• Full analysis

Parameters analysed by Spermalyze

The system automatically analyses all critical semen quality parameters. It is the first system on the market to detect agglutination grade, detached heads and DMR percentage within the analysis itself.

Motility and kinematics

  • Total and progressive motility
  • DCL · DSL · DAP
  • VCL · VSL · VAP
  • STR · LIN · WOB
  • ALH · BCF · MAD

Cell-by-cell tracking · Speed classification: fast (green), medium (blue), slow (yellow), static (red)

Morphology and concentration

  • Concentration · Functional concentration
  • % Proximal droplets
  • % Distal droplets
  • % Coiled tails
  • % Coiled flagella
  • % DRM — distal reflex midpiece
  • % Detached heads
  • % Motile and morphologically normal spermatozoa
  • % Agglutination grade

First system on the market with automatic detection of agglutination grade, DMR and detached heads

Configuration

Two options: manual and automatic

The heated stage microscope with 5 MP camera is the core of the system. The AS-10 automatic stage adds autonomy and throughput for high-volume laboratories.

Manual heated stage

Standard configuration
 
  • Heated stage at 37°C
  • 5 MP real camera
  • Field-by-field manual analysis
  • Results in under 2 minutes per sample
  • Compatible with multiple microscope models
  • Ideal for moderate-volume laboratories

AS-10 Automatic stage | OPCIONAL

High throughput
 
  • Fully integrated with microscope and software
  • Automatic movement between wells
  • Autofocus of each field in each well
  • Up to 20 samples in 5 minutes with a single click
  • Sequential analysis without operator intervention
  • Ideal for high-volume production centres

Traceability

Organised data, ready to analyse

Spermalyze includes a data manager that stores information on animals, centres, operators and doses. Filter, compare and export everything from a single interface.

Centralised database
Stores photos, videos and reports of all samples. Links initial analyses with subsequent quality controls.

Video re-analysis
Re-analyse past videos by changing parameters without repeating the work at the microscope.

Excel and PDF export
Clean, structured data ready for statistical analysis, publications and thesis preparation.
Integrable with your systems
The management system can be linked to other databases and management software already in use at your centre.

Workflow

01

Prepare the sample
Dilute the ejaculate and load the counting chamber. Microscope at 37°C.

02

Capture and analyse
The software captures fields and classifies each spermatozoon. Results in under 2 minutes.

03

Review the results
View the report, add labels or notes if needed.

04

Export and document
Excel or PDF with complete kinetic data, clean and structured for statistical analysis.

Evidence

Tested in real laboratory environments

Spermalyze has been developed and validated with real bovine, equine and canine samples at European research and production centres. Its algorithms have been trained and adjusted under real conditions, not idealised laboratory environments.

R²=0,99

Concentration correlation vs NucleoCounter®, the sector gold standard

65+

Frozen-thawed semen samples evaluated in morphology validation
 

50

Videos analysed in subjective validation of abnormal form detection
 

Concentration

Validated with fresh and frozen-thawed semen against NucleoCounter® and another commercial CASA system. Spermalyze obtains values closer to the reference standard across all evaluated concentrations.

Motility

High correlation for total and progressive motility in fresh semen. Good correlation values in post-thaw samples, where sample variability is inherently higher.

Morphology

Compared against the average of 5 specialist technicians using eosin/nigrosine stained smears. More precise results in classification of coiled tails and DMR compared to the manual method.

Repeatability

Very low deviations when analysing two aliquots from the same tube in both fresh and frozen-thawed semen. Indicator of high system consistency between consecutive analyses.

 

SUPPORT

More than software: end-to-end support

Beyond the system itself, we provide installation, training and ongoing technical support to ensure Spermalyze integrates smoothly into your laboratory routine from day one.

Installation and commissioning

We handle hardware installation and software configuration tailored to your workflow and species of work.

 

Team training

We train your team in system operation, result interpretation and configuration of quality alerts and routines.

Ongoing technical support

Support throughout the entire project lifecycle: parameter adjustment, incident resolution and system updates.

More solutions

Spermalyze is just the beginning

Precisión Celular develops further cell and semen analysis solutions for different working contexts — from field to research laboratory, and from closed systems to fully custom development.

Field analysis

Portable device

Semen quality evaluation outside the laboratory, at the moment of collection or before insemination.

Equipment

Microscopy and accessories

Microscopes, cameras and heating plates compatible with our analysis systems.

R&D

Custom development

Algorithms and solutions for new species, custom protocols or integration with your current management systems.

NEXT STEP

Want to see Spermalyze

in action?

We can review your case together and show you how Spermalyze works in a real laboratory environment, with samples from your species and your usual protocol.

Or write to us at conecta@precisioncelular.com